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Impact of l -lactate metabolism on HDM-induced murine airway inflammation and Th2 immune response. (A) LDH enzyme activity detection in <t>serum</t> (left) and lung tissue homogenates (right) of <t>mice</t> in each group. (B) Quantitative analysis of lactate concentrations in serum and lung tissue. (C) Diff-Quik staining images of BALF cell smears (left, red box indicates magnified field) and statistics of eosinophil proportions (right). (D) H&E staining (top, showing inflammatory infiltration) and PAS staining (bottom, showing goblet cells and mucus) of lung tissue sections. Red arrows indicate lesion areas. Right side shows pathological scores. (E) ELISA detection of IL-4, IL-5, and IL-13 concentrations in BALF. (F) ELISA detection of serum total <t>IgE</t> and HDM-specific IgE levels. (G) Flow cytometric analysis of Th1 (IFN-γ + CD4 + ) and Th2 (IL-4 + CD4 + ) cell subsets in mediastinal lymph nodes (mLN) and spleen. Data are expressed as Mean ± SEM. n = 10 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant (One-way ANOVA).
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Impact of l -lactate metabolism on HDM-induced murine airway inflammation and Th2 immune response. (A) LDH enzyme activity detection in <t>serum</t> (left) and lung tissue homogenates (right) of <t>mice</t> in each group. (B) Quantitative analysis of lactate concentrations in serum and lung tissue. (C) Diff-Quik staining images of BALF cell smears (left, red box indicates magnified field) and statistics of eosinophil proportions (right). (D) H&E staining (top, showing inflammatory infiltration) and PAS staining (bottom, showing goblet cells and mucus) of lung tissue sections. Red arrows indicate lesion areas. Right side shows pathological scores. (E) ELISA detection of IL-4, IL-5, and IL-13 concentrations in BALF. (F) ELISA detection of serum total <t>IgE</t> and HDM-specific IgE levels. (G) Flow cytometric analysis of Th1 (IFN-γ + CD4 + ) and Th2 (IL-4 + CD4 + ) cell subsets in mediastinal lymph nodes (mLN) and spleen. Data are expressed as Mean ± SEM. n = 10 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant (One-way ANOVA).
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Impact of l -lactate metabolism on HDM-induced murine airway inflammation and Th2 immune response. (A) LDH enzyme activity detection in <t>serum</t> (left) and lung tissue homogenates (right) of <t>mice</t> in each group. (B) Quantitative analysis of lactate concentrations in serum and lung tissue. (C) Diff-Quik staining images of BALF cell smears (left, red box indicates magnified field) and statistics of eosinophil proportions (right). (D) H&E staining (top, showing inflammatory infiltration) and PAS staining (bottom, showing goblet cells and mucus) of lung tissue sections. Red arrows indicate lesion areas. Right side shows pathological scores. (E) ELISA detection of IL-4, IL-5, and IL-13 concentrations in BALF. (F) ELISA detection of serum total <t>IgE</t> and HDM-specific IgE levels. (G) Flow cytometric analysis of Th1 (IFN-γ + CD4 + ) and Th2 (IL-4 + CD4 + ) cell subsets in mediastinal lymph nodes (mLN) and spleen. Data are expressed as Mean ± SEM. n = 10 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant (One-way ANOVA).
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Impact of l -lactate metabolism on HDM-induced murine airway inflammation and Th2 immune response. (A) LDH enzyme activity detection in <t>serum</t> (left) and lung tissue homogenates (right) of <t>mice</t> in each group. (B) Quantitative analysis of lactate concentrations in serum and lung tissue. (C) Diff-Quik staining images of BALF cell smears (left, red box indicates magnified field) and statistics of eosinophil proportions (right). (D) H&E staining (top, showing inflammatory infiltration) and PAS staining (bottom, showing goblet cells and mucus) of lung tissue sections. Red arrows indicate lesion areas. Right side shows pathological scores. (E) ELISA detection of IL-4, IL-5, and IL-13 concentrations in BALF. (F) ELISA detection of serum total <t>IgE</t> and HDM-specific IgE levels. (G) Flow cytometric analysis of Th1 (IFN-γ + CD4 + ) and Th2 (IL-4 + CD4 + ) cell subsets in mediastinal lymph nodes (mLN) and spleen. Data are expressed as Mean ± SEM. n = 10 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant (One-way ANOVA).
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Impact of l -lactate metabolism on HDM-induced murine airway inflammation and Th2 immune response. (A) LDH enzyme activity detection in <t>serum</t> (left) and lung tissue homogenates (right) of <t>mice</t> in each group. (B) Quantitative analysis of lactate concentrations in serum and lung tissue. (C) Diff-Quik staining images of BALF cell smears (left, red box indicates magnified field) and statistics of eosinophil proportions (right). (D) H&E staining (top, showing inflammatory infiltration) and PAS staining (bottom, showing goblet cells and mucus) of lung tissue sections. Red arrows indicate lesion areas. Right side shows pathological scores. (E) ELISA detection of IL-4, IL-5, and IL-13 concentrations in BALF. (F) ELISA detection of serum total <t>IgE</t> and HDM-specific IgE levels. (G) Flow cytometric analysis of Th1 (IFN-γ + CD4 + ) and Th2 (IL-4 + CD4 + ) cell subsets in mediastinal lymph nodes (mLN) and spleen. Data are expressed as Mean ± SEM. n = 10 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant (One-way ANOVA).
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Impact of l -lactate metabolism on HDM-induced murine airway inflammation and Th2 immune response. (A) LDH enzyme activity detection in <t>serum</t> (left) and lung tissue homogenates (right) of <t>mice</t> in each group. (B) Quantitative analysis of lactate concentrations in serum and lung tissue. (C) Diff-Quik staining images of BALF cell smears (left, red box indicates magnified field) and statistics of eosinophil proportions (right). (D) H&E staining (top, showing inflammatory infiltration) and PAS staining (bottom, showing goblet cells and mucus) of lung tissue sections. Red arrows indicate lesion areas. Right side shows pathological scores. (E) ELISA detection of IL-4, IL-5, and IL-13 concentrations in BALF. (F) ELISA detection of serum total <t>IgE</t> and HDM-specific IgE levels. (G) Flow cytometric analysis of Th1 (IFN-γ + CD4 + ) and Th2 (IL-4 + CD4 + ) cell subsets in mediastinal lymph nodes (mLN) and spleen. Data are expressed as Mean ± SEM. n = 10 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant (One-way ANOVA).
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Impact of l -lactate metabolism on HDM-induced murine airway inflammation and Th2 immune response. (A) LDH enzyme activity detection in <t>serum</t> (left) and lung tissue homogenates (right) of <t>mice</t> in each group. (B) Quantitative analysis of lactate concentrations in serum and lung tissue. (C) Diff-Quik staining images of BALF cell smears (left, red box indicates magnified field) and statistics of eosinophil proportions (right). (D) H&E staining (top, showing inflammatory infiltration) and PAS staining (bottom, showing goblet cells and mucus) of lung tissue sections. Red arrows indicate lesion areas. Right side shows pathological scores. (E) ELISA detection of IL-4, IL-5, and IL-13 concentrations in BALF. (F) ELISA detection of serum total <t>IgE</t> and HDM-specific IgE levels. (G) Flow cytometric analysis of Th1 (IFN-γ + CD4 + ) and Th2 (IL-4 + CD4 + ) cell subsets in mediastinal lymph nodes (mLN) and spleen. Data are expressed as Mean ± SEM. n = 10 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant (One-way ANOVA).
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Impact of l -lactate metabolism on HDM-induced murine airway inflammation and Th2 immune response. (A) LDH enzyme activity detection in serum (left) and lung tissue homogenates (right) of mice in each group. (B) Quantitative analysis of lactate concentrations in serum and lung tissue. (C) Diff-Quik staining images of BALF cell smears (left, red box indicates magnified field) and statistics of eosinophil proportions (right). (D) H&E staining (top, showing inflammatory infiltration) and PAS staining (bottom, showing goblet cells and mucus) of lung tissue sections. Red arrows indicate lesion areas. Right side shows pathological scores. (E) ELISA detection of IL-4, IL-5, and IL-13 concentrations in BALF. (F) ELISA detection of serum total IgE and HDM-specific IgE levels. (G) Flow cytometric analysis of Th1 (IFN-γ + CD4 + ) and Th2 (IL-4 + CD4 + ) cell subsets in mediastinal lymph nodes (mLN) and spleen. Data are expressed as Mean ± SEM. n = 10 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant (One-way ANOVA).

Journal: Redox Biology

Article Title: Lactate-driven ATP6V1B2 lactylation triggers asthmatic inflammation by linking lysosomal dysfunction to mitochondrial ROS-dependent pyroptosis

doi: 10.1016/j.redox.2026.104059

Figure Lengend Snippet: Impact of l -lactate metabolism on HDM-induced murine airway inflammation and Th2 immune response. (A) LDH enzyme activity detection in serum (left) and lung tissue homogenates (right) of mice in each group. (B) Quantitative analysis of lactate concentrations in serum and lung tissue. (C) Diff-Quik staining images of BALF cell smears (left, red box indicates magnified field) and statistics of eosinophil proportions (right). (D) H&E staining (top, showing inflammatory infiltration) and PAS staining (bottom, showing goblet cells and mucus) of lung tissue sections. Red arrows indicate lesion areas. Right side shows pathological scores. (E) ELISA detection of IL-4, IL-5, and IL-13 concentrations in BALF. (F) ELISA detection of serum total IgE and HDM-specific IgE levels. (G) Flow cytometric analysis of Th1 (IFN-γ + CD4 + ) and Th2 (IL-4 + CD4 + ) cell subsets in mediastinal lymph nodes (mLN) and spleen. Data are expressed as Mean ± SEM. n = 10 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant (One-way ANOVA).

Article Snippet: Mouse serum total IgE and HDM-specific IgE (R&D Systems), as well as IL-4 (E-EL-M0043), IL-5 (E-EL-M0722), IL-13 (E-EL-M0727), HMGB1 (E-EL-M0676/E-EL-H1554), IL-1β (E-EL-H0149), and IL-18 (E-EL-H0253) in BALF or cell supernatants were all detected using ELISA kits from Elabscience.

Techniques: Activity Assay, Diff-Quik, Staining, Enzyme-linked Immunosorbent Assay

Effect of blocking ATP6V1B2 lactylation on mouse asthma phenotypes and in vivo pyroptosis signals. (A) H&E and PAS staining and scoring of AAV-transduced mouse lung tissues. (B) Flow cytometric analysis of eosinophils (CD11C − CD45.2 + Siglec-F + ) in BALF. (C) Detection of serum total IgE and HDM-specific IgE levels. (D) ELISA analysis of Th2 cytokines (IL-4, IL-5, IL-13) in BALF. (E) Measurement of lactate levels in serum and lung tissue homogenates. (F) Immunofluorescence staining of in situ Pan-Kla (green) and LAMP1 (red) in lung tissue frozen sections. Scale bar: 100 μm. (G) Immunoblot analysis of GSDME and Caspase-3 cleavage in lung tissue. (H) Lung tissue LDH activity detection. Data are expressed as Mean ± SEM. n = 10 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant.

Journal: Redox Biology

Article Title: Lactate-driven ATP6V1B2 lactylation triggers asthmatic inflammation by linking lysosomal dysfunction to mitochondrial ROS-dependent pyroptosis

doi: 10.1016/j.redox.2026.104059

Figure Lengend Snippet: Effect of blocking ATP6V1B2 lactylation on mouse asthma phenotypes and in vivo pyroptosis signals. (A) H&E and PAS staining and scoring of AAV-transduced mouse lung tissues. (B) Flow cytometric analysis of eosinophils (CD11C − CD45.2 + Siglec-F + ) in BALF. (C) Detection of serum total IgE and HDM-specific IgE levels. (D) ELISA analysis of Th2 cytokines (IL-4, IL-5, IL-13) in BALF. (E) Measurement of lactate levels in serum and lung tissue homogenates. (F) Immunofluorescence staining of in situ Pan-Kla (green) and LAMP1 (red) in lung tissue frozen sections. Scale bar: 100 μm. (G) Immunoblot analysis of GSDME and Caspase-3 cleavage in lung tissue. (H) Lung tissue LDH activity detection. Data are expressed as Mean ± SEM. n = 10 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant.

Article Snippet: Mouse serum total IgE and HDM-specific IgE (R&D Systems), as well as IL-4 (E-EL-M0043), IL-5 (E-EL-M0722), IL-13 (E-EL-M0727), HMGB1 (E-EL-M0676/E-EL-H1554), IL-1β (E-EL-H0149), and IL-18 (E-EL-H0253) in BALF or cell supernatants were all detected using ELISA kits from Elabscience.

Techniques: Blocking Assay, In Vivo, Staining, Enzyme-linked Immunosorbent Assay, Immunofluorescence, In Situ, Western Blot, Activity Assay